Euro 2016: English referee Martin Atkinson for Northern Ireland v Wales
The all-British last-16 tie will be Atkinson's third game of this summer's championships after he officiated Germany's victory over Ukraine and Portugal's thrilling 3-3 draw against Hungary on Wednesday.
Mark Clattenburg will also feature in the first round of the knock-out stages.
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Hide AdEnglishman Clattenburg, who took charge of last month's FA Cup final as well as the all-Madrid Champions League showpiece, has been handed Switzerland's match against Poland on Saturday.
Clattenburg oversaw Italy's win against Belgium and the Czech Republic's 2-2 draw with Croatia which was marred by crowd trouble.